Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p339 | (1) | WCRB2014

Hypothalamic arcuate nuleus-specific enhancer for kisspeptin expression of female mice

Goto Teppei , Tsukamura Hiroko , Tomikawa Junko , Abe Hitomi , Fukanuma Tatsuya , Imaura Takuya , Takase Kenji , Sanbo Makoto , Tomita Koichi , Hirabayashi Masumi , Maeda Kei-ichiro , Uenoyama Yoshihisa

Two populations of kisspeptin neurons, located in the arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV), are considered to be involved in generating GnRH pulse and surge respectively. The present study aimed to determine the region-specific enhancer for ARC Kiss1 gene expression by in vivo reporter assay using transgenic (Tg) mice. Three GFP reporter constructs (long-, middle-, and short-length) were generated by insertion of GFP cDNA in ...